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Pavadinimas anglų kalba: Mechanisms and Methods of Bioelectrotechnologies Dalyko aprašo rengėjas -ai : Prof. Gintautas Saulis, Dr. Saulė,Department of Biology, Faculty 1 btc į crc Natural Sciences, Vytautas Magnus University Dalyko anotacija lietuvių kalba Kurso metu susipažįstama su šiuolaikinių bioelectrotechnologijų pagrindą sudarančių reiškinių mechanizmais.

Be gilaus teorinio šių technologijų supratimo, studentai įgis naudingų praktinių įgūdžių, būtinų norint sėkmingai taikyti šias technologijas. Dalyko anotacija užsienio kalba The course introduces to the fundamental basis of innovative bioelectrotechnologies.

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During this course besides a deep theoretical understanding, a lot of practical knowledge, which is necessary for successful application of these techniques, will be obtained. Dalyko studijų rezultatai Understand the origin of bioelectrical potentials, mechanims of generation of the transmembrane potential by the external electric field.

Understand time-course of the transmembrane potential and the influence of various 1 btc į crc on the transmembrane potential. Understand the mechanims of basic electrokinetic phenomenon. Understand the mechanims of the phenomena underlying innovative bioelectrotechnologies.

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Acquire the skills in using various methods of studying bioelectrical potentials, basic cell electroporation phenomenon electroporation, electropermeabilization, cell death. Dalyko turinys 1. History of Bioelectrotechnologies electroporation, electrofusion, electroinsertion, electrotransformation, etc.

Electrokinetics Phenomena electrodeformation, electroorientation, electrorotation, dielectrophoresis and their Applications in Biotechnology 5. Origin of bioelectric potential diffusion potential, transmembrane potential, Nerst equation, Goldman equation, distribution of the electric potential at the interface of two phases.

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Transmembrane potential created by the external electric field conductivity- and capacity-coupling. Mechanims of cell electroporation, electropermeabilization, and death induced by pulsed electrid fields. Mechanims of cell electrofusion, electroinsertion of proteins, electrochemotherapy.

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Mechanims of electrically induced gene transfer, transdermal drug delivery, electroporation during electrostimulation and defibrillation, ablation by irreversible electroporation.

Applications of of Innovative Bioelectrotechnologies Practical work contents : Dielectrophoresis.

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Estimation of the influence of the medium conductivity on the cell transmembrane potential. Determination of the cell membrane electroporation from the release of intercellular potassium ions.

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Determination of cell electropermeabilization for bleomycin. Cell death induced by pulsed electrid fields. Modeling of the response of the cells to pulses of different shape unipolar square-wave, bypolar square-wave, exponential, sine-wave.

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Cell electrofusion. Dalyko studijos valandomis Lectures — 30 hours, laboratory work — 30 hours, individual work — 94 hours, examinations— 6 hours. Total h. Literatūra 1.

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Glazer, Biophysics. Berlin: Springer Verlag 3. Malmivuo and R. Pakhomov, D. Miklavcic, and M. Markov Eds. Enrico P. Spugnini and Alfonso Baldi Eds.

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